WB
Western blot analysis of various lysates using Pan-Crotonyl-K Rabbit pAb at 1:1000 dilution. HeLa and NIH/3T3 cells were treated with Sodium crotonate for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.WB
Western blot analysis of various lysates using Pan-Crotonyl-K Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.| Product Name | Pan-Crotonyl-K Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Lysine crotonylation (Kcr) was first discovered about 10 years ago, as a PTM of histone. It was revealed that Kcr is an evolutionarily conserved and common PTM that occurs in both core histone and some non-histone proteins in a variety of organisms. Similar to other types of PTM, Kcr is a reversible modification. The classic histone acetyltransferases (HATs), p300/CBP, PCAF, and MOF, are responsible for most crotonylation events, while the histone deacetylases (HDACs) HDAC1/2/3 and SIRT1/2/3 reverse these reactions . The chromodomain protein CDYL acts as a crotonyl-CoA hydratase to negatively regulate histone Kcr by reducing substrate supply. CDYL-regulated Kcr of RPA1 plays an important role in homologous recombination (HR)-mediated DNA repair (23), while HDAC-regulated histone crotonylation is reduced after DNA damage . |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 10-250kDa |
|---|
WB
Western blot analysis of various lysates using Pan-Crotonyl-K Rabbit pAb at 1:1000 dilution. HeLa and NIH/3T3 cells were treated with Sodium crotonate for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
WB
Western blot analysis of various lysates using Pan-Crotonyl-K Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
