WB
Western blot analysis of lysates from mouse muscle, using CNTF Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.ICC/IF
Immunofluorescence analysis of C6 cells using CNTF Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IF-P
Immunofluorescence analysis of paraffin embedded mouse spinal cord using CNTF Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | CNTF Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CNTF |
|---|---|
| Gene Synonyms | HCNTF |
| Gene Full Name | ciliary neurotrophic factor |
| Gene Summary | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| Alternative Names | HCNTF CNTF |
| Molecular Weight(MW) | 23kDa(Observed MW 26kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of lysates from mouse muscle, using CNTF Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
ICC/IF
Immunofluorescence analysis of C6 cells using CNTF Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IF-P
Immunofluorescence analysis of paraffin embedded mouse spinal cord using CNTF Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
