WB
Western blot analysis of various lysates using CELF5 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μ g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 180s.ICC/IF
mmunofluorescence analysis of L929 cells using CELF5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | CELF5 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human FGFR1 (NP_075598.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 ELISA:Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CELF5 |
|---|---|
| Gene Synonyms | CELF-5 BRUNOL5 BRUNOL-5 CELF5 |
| Gene Full Name | CUGBP Elav-like family member 5 |
| Gene Summary | This gene encodes a member of the the CELF/BRUNOL protein family, which contain two Nterminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing and translation. Alternatively spliced transcript variants have been found for this gene. |
| Alternative Names | CELF-5 BRUNOL5 BRUNOL-5 CELF5 |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of various lysates using CELF5 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μ g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 180s.
ICC/IF
mmunofluorescence analysis of L929 cells using CELF5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3- conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 ELISA:Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
