WB
L02 cells were subjected to SDS PAGE followed by western blot with HNF1B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.WB
MDCK cells were subjected to SDS PAGE followed by western blot with HNF1B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HNF1B antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using HNF1B antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed L02 cells using HNF1B antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | HNF1B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human HNF1B protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:200-1:800 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HNF1B |
|---|---|
| Gene Synonyms | T2D FJHN HNF2 LFB3 RCAD TCF2 HPC11 LF-B3 MODY5 TCF-2 VHNF1 ADTKD3 HNF-1B HNF1beta HNF-1-beta |
| Gene Full Name | HNF1 homeobox B |
| Gene Summary | This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Alternative Names | TCF-2 TCF2 HNF-1-beta HNF-1B HNF 1 beta |
| Molecular Weight(MW) | 61kDa |
| Cellular Localization | Nucleus. |
WB
L02 cells were subjected to SDS PAGE followed by western blot with HNF1B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB
MDCK cells were subjected to SDS PAGE followed by western blot with HNF1B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HNF1B antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using HNF1B antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed L02 cells using HNF1B antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:1000 IHC:1:200-1:800 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
