WB
Western blot analysis of Myogenin on different lysates with Rabbit anti-Myogenin antibody at 1/1,000 dilution. Lane 1: Undifferentiated C2C12 whole cell lysate, Lane 2: Differentiated C2C12 for 1 day whole cell lysate, Lane 3: Differentiated C2C12 for 2 days whole cell lysate, Lane 4: Differentiated C2C12 for 3 days whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IF-F
Species: Mouse, Site: embryo, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.IF-F
Species: Rat, Site: embryo, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.| Product Name | Myogenin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Myogenin aa 1-50 / 224. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FWB |
| WB:1:1000 IF-F:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYOG |
|---|---|
| Gene Synonyms | MYF4 myf-4 bHLHc3 |
| Gene Full Name | myogenin |
| Gene Summary | Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008] |
| Alternative Names | bHLHc3 antibody cb553 antibody Class C basic helix-loop-helix protein 3 antibody MYF 4 antibody Myf-4 antibody MYF4 antibody MYOG antibody MYOG_HUMAN antibody Myogenic factor 4 antibody Myogenic factor 4 myogenin antibody bHLHc3 antibody cb553 antibody Class C basic helix-loop-helix protein 3 antibody MYF 4 antibody Myf-4 antibody MYF4 antibody MYOG antibody MYOG_HUMAN antibody Myogenic factor 4 antibody Myogenic factor 4 myogenin antibody Myogenin antibody Myogenin myogenin factor 4 antibody OTTHUMP00000039094 antibody |
| Molecular Weight(MW) | 25 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of Myogenin on different lysates with Rabbit anti-Myogenin antibody at 1/1,000 dilution. Lane 1: Undifferentiated C2C12 whole cell lysate, Lane 2: Differentiated C2C12 for 1 day whole cell lysate, Lane 3: Differentiated C2C12 for 2 days whole cell lysate, Lane 4: Differentiated C2C12 for 3 days whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IF-F
Species: Mouse, Site: embryo, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.
IF-F
Species: Rat, Site: embryo, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.| Application Notes | WB:1:1000 IF-F:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
