WB
Western blot analysis of Myogenin on different lysates with Mouse anti-Myogenin antibody at 1/2,000 dilution. Lane 1: RD cell lysate, Lane 2: HeLa cell lysate (negative), Lane 3: C2C12 cell lysate, Lane 4: C2C12 treated with 2% horse serum for 24 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Mouse anti-Myogenin antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of RD (positive) and HeLa (negative) labeling Myogenin with Mouse anti-Myogenin antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myogenin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.IF-F
Species: Rat, Site: embryo, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.| Product Name | Myogenin Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIF-FIHCWB |
| WB:1:2000 IHC:1:500 ICC/IF:1:100 IF-F:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYOG |
|---|---|
| Gene Synonyms | MYF4 myf-4 bHLHc3 |
| Gene Full Name | myogenin |
| Gene Summary | Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008] |
| Alternative Names | bHLHc3 antibody cb553 antibody Class C basic helix-loop-helix protein 3 antibody MYF 4 antibody Myf-4 antibody MYF4 antibody MYOG antibody MYOG_HUMAN antibody Myogenic factor 4 antibody Myogenic factor 4 myogenin antibody bHLHc3 antibody cb553 antibody Class C basic helix-loop-helix protein 3 antibody MYF 4 antibody Myf-4 antibody MYF4 antibody MYOG antibody MYOG_HUMAN antibody Myogenic factor 4 antibody Myogenic factor 4 myogenin antibody Myogenin antibody Myogenin myogenin factor 4 antibody OTTHUMP00000039094 antibody |
| Molecular Weight(MW) | 25 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of Myogenin on different lysates with Mouse anti-Myogenin antibody at 1/2,000 dilution. Lane 1: RD cell lysate, Lane 2: HeLa cell lysate (negative), Lane 3: C2C12 cell lysate, Lane 4: C2C12 treated with 2% horse serum for 24 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Mouse anti-Myogenin antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of RD (positive) and HeLa (negative) labeling Myogenin with Mouse anti-Myogenin antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myogenin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.
IF-F
Species: Rat, Site: embryo, Sample: Frozen section, Antibody concentration: 1/200, Antigen retrieval: Not required.| Application Notes | WB:1:2000 IHC:1:500 ICC/IF:1:100 IF-F:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.. |
