WB
Western blot analysis of HNF4A using anti-HNF4A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNF4A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to Zebrafish HNF4A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish HNF4A recombinant protein (Position: G28-M336). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | hnf4a |
|---|---|
| Gene Synonyms | fb58h09 sb:eu665 id:ibd1279 wu:fb58h09 |
| Gene Full Name | hepatocyte nuclear factor 4, alpha |
| Gene Summary | Enables DNA-binding transcription factor activity. Acts upstream of or within pancreas regeneration; response to bacterium; and type B pancreatic cell development. Predicted to be located in nucleus. Is expressed in gut; intestinal bulb primordium; liver; pleuroperitoneal region; and yolk syncytial layer. Human ortholog(s) of this gene implicated in Fanconi renotubular syndrome 4; familial combined hyperlipidemia; hypoglycemia; maturity-onset diabetes of the young type 1; and type 2 diabetes mellitus. Orthologous to human HNF4A (hepatocyte nuclear factor 4 alpha). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | HNF4a9 HNF4a8 HNF4a7 HNF4 HNF 4 alpha |
| Molecular Weight(MW) | 47 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of HNF4A using anti-HNF4A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNF4A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
