WB
Sample: Lane 1: Recombinant HPV16 E6 protein, DsbC & His 0.1ug, Lane 2: Recombinant HPV16 E6 protein, DsbC & His0.05ug Primary: Anti-HPV16 E6 + HPV18 E6 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Tissue: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB stainingFC
Blank control（black line）:Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 + HPV18 E6 antibody, Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | HPV16 E6 + HPV18 E6 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from HPV16 E6+ HPV18 E6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 FC:1ug/Test |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | E6 |
|---|---|
| Gene Synonyms | protein E6* transforming protein E6 HpV16gp1 |
| Target Description | HPV16, HPV18 |
| Alternative Names | HPV16+HPV18 E6 HPV16/18 protein Human Papilloma virus E6 Human papillomavirus type 16 E6 Human papillomavirus type 18 E6 Protein E6 . |
| Molecular Weight(MW) | 11kDa(Observed band size: 50 kDa) |
WB
Sample: Lane 1: Recombinant HPV16 E6 protein, DsbC & His 0.1ug, Lane 2: Recombinant HPV16 E6 protein, DsbC & His0.05ug Primary: Anti-HPV16 E6 + HPV18 E6 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Tissue: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
FC
Blank control（black line）:Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 + HPV18 E6 antibody, Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 FC:1ug/Test |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
