WB
Sample: Lane 1: HPV16 E6 fusion protein 0.1ug Lysates, Lane 2: HPV16 E6 fusion protein 0.05ug Lysates, Primary: Anti-HPV16 E6 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HPV16 E6) Polyclonal Antibody at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.FC
Blank control（black line）:Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 antibody, Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488, Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG, Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | HPV16 E6 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant HPV16 E6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 FC:1ug/Test |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | E6 |
|---|---|
| Gene Synonyms | protein E6* transforming protein E6 HpV16gp1 |
| Target Description | HPV16 |
| Alternative Names | E6 HPV16 E6 protein Human Papilloma Virus Human papillomavirus type 16 E6 Human papillomavirus type 16 Protein E6 E6 protein HPV16 E6 HPV16-E6 VE6_HPV16. |
| Molecular Weight(MW) | 11kDa(Observed band size: 50 kDa) |
WB
Sample: Lane 1: HPV16 E6 fusion protein 0.1ug Lysates, Lane 2: HPV16 E6 fusion protein 0.05ug Lysates, Primary: Anti-HPV16 E6 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HPV16 E6) Polyclonal Antibody at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
FC
Blank control（black line）:Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 antibody, Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488, Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG, Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 FC:1ug/Test |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
