WB
Western blot analysis of Collagen 14 alpha 1 expression in human lung (A) whole cell lysates.IHC
Immunohistochemical analysis of Collagen 14 alpha 1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.FC
Flow cytometric analysis of Hela cells using Anti-Collagen 14 alpha 1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.| Product Name | Anti-Collagen 14 alpha 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human Collagen 14 alpha 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:100 FC:1:10-1:30 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL14A1 |
|---|---|
| Gene Synonyms | UND |
| Gene Full Name | collagen type XIV alpha 1 chain |
| Gene Summary | This gene encodes the alpha chain of type XIV collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XIV collagen interacts with the fibril surface and is involved in the regulation of fibrillogenesis. [provided by RefSeq, Jan 2013] |
| Alternative Names | UND Collagen alpha-1(XIV) chain Undulin |
| Molecular Weight(MW) | 193 kDa(Observed band size: 250 kDa) |
WB
Western blot analysis of Collagen 14 alpha 1 expression in human lung (A) whole cell lysates.
IHC
Immunohistochemical analysis of Collagen 14 alpha 1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system.. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
FC
Flow cytometric analysis of Hela cells using Anti-Collagen 14 alpha 1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:100 FC:1:10-1:30 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
