WB
Western blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS CoV-2 NSP1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IP
Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg SARS-CoV-2 NSP1 antibody. Western blot was performed from the immunoprecipitate using SARS-CoV-2 NSP1 antibody at a dilution of 1:1000.| Product Name | SARS-CoV-2 NSP1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:500-1:1000 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ORF1ab |
|---|---|
| Gene Summary | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies. |
| Molecular Weight(MW) | 141 kDa(Observed MW 20 kDa) |
WB
Western blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS CoV-2 NSP1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IP
Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg SARS-CoV-2 NSP1 antibody. Western blot was performed from the immunoprecipitate using SARS-CoV-2 NSP1 antibody at a dilution of 1:1000.| Application Notes | WB:1:500-1:1000 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
