WB
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Rat heart tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibod at 1:100,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate, Lane 2: Rat skeletal muscle tissue lysate, Lane 3: Mouse smooth muscle tissue lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.| Product Name | MT-ND1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human MT-ND1 aa 23-67 / 318. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MT-ND1 |
|---|---|
| Gene Synonyms | MTND1 ND1 |
| Gene Full Name | mitochondrially encoded NADH dehydrogenase 1 |
| Gene Summary | Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Located in mitochondrial inner membrane. Part of respiratory chain complex I. Implicated in several diseases, including MELAS syndrome; neurodegenerative disease (multiple); optic nerve disease (multiple); toxic shock syndrome; and type 2 diabetes mellitus. Biomarker of Alzheimer's disease; Parkinson's disease; and multiple sclerosis. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Complex I subunit ND1 antibody Mitochondrially encoded NADH dehydrogenase 1 antibody MT-ND1 antibody MTND1 antibody NAD1 antibody NADH dehydrogenase subunit 1 (complex I) antibody NADH dehydrogenase subunit 1 antibody NADH-ubiquinone oxidoreductase chain 1 antibody NADH-ubiquinone oxidoreductase subunit ND1 antibody NADH1 antibody Complex I subunit ND1 antibody Mitochondrially encoded NADH dehydrogenase 1 antibody MT-ND1 antibody MTND1 antibody NAD1 antibody NADH dehydrogenase subunit 1 (complex I) antibody NADH dehydrogenase subunit 1 antibody NADH-ubiquinone oxidoreductase chain 1 antibody NADH-ubiquinone oxidoreductase subunit ND1 antibody NADH1 antibody ND1 antibody NU1M_HUMAN antibody |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Mitochondrion inner membrane. |
WB
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Rat heart tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibod at 1:100,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate, Lane 2: Rat skeletal muscle tissue lysate, Lane 3: Mouse smooth muscle tissue lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
