WB
Gel: 8%SDS-PAGE Lysate: 35μg Lane 1-2: H2228 and A549 cell lysates Primary antibody: ALK Antibody at dilution 1/1000 Secondary antibody: Goat anti mouse IgG at 1/8000 dilution Exposure time: 15 minutesIHC
The image is immunohistochemistry of paraffin-embedded human non-small cell lung cancer sample with ALK translocation detected by FISH, (Original magnification: ×200)| Product Name | Anti-ALK mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion protein of human ALK |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALK |
|---|---|
| Gene Synonyms | ALK1 CD246 NBLST3 |
| Gene Full Name | ALK receptor tyrosine kinase |
| Gene Summary | This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011] |
| Alternative Names | CD246 NBLST3 |
| Molecular Weight(MW) | 176 kDa (Observed MW 95 kDa) |
| Cellular Localization | Cell membrane,Membrane |
WB
Gel: 8%SDS-PAGE Lysate: 35μg Lane 1-2: H2228 and A549 cell lysates Primary antibody: ALK Antibody at dilution 1/1000 Secondary antibody: Goat anti mouse IgG at 1/8000 dilution Exposure time: 15 minutes
IHC
The image is immunohistochemistry of paraffin-embedded human non-small cell lung cancer sample with ALK translocation detected by FISH, (Original magnification: ×200)| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
