WB
Western blot analysis IFI27 using Jurkat whole cell lysates.IHC
IFI27 Antibody at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.IHC
IFI27 Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to IFI27 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human IFI27, corresponding to a region within N-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFI27 |
|---|---|
| Gene Synonyms | P27 ISG12 FAM14D ISG12A |
| Gene Full Name | interferon alpha inducible protein 27 |
| Gene Summary | Enables several functions, including RNA polymerase II-specific DNA-binding transcription factor binding activity; identical protein binding activity; and lamin binding activity. Involved in several processes, including defense response to other organism; protein K48-linked ubiquitination; and pyroptotic inflammatory response. Acts upstream of or within negative regulation of transcription by RNA polymerase II and regulation of protein export from nucleus. Located in mitochondrial membrane and nuclear inner membrane. Is active in mitochondrion. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | 2310061N23Rik FAM 14D FAM14D IFI 27 IFI27 IFI27_HUMAN Interferon alpha induced 11.5 kDa protein Interferon alpha inducible protein 27 Interferon alpha-induced 11.5 kDa protein Interferon alpha-inducible protein 27 interferon alpha-inducible protein 27 mitochondrial Interferon inducible protein 27 interferon-stimulated gene 12 Interferon-stimulated gene 12a protein ISG 12 ISG12 ISG12(a) ISG12(a) protein ISG12A mitochondrial p27 |
| Molecular Weight(MW) | 12 kDa(Observed band size: 11 kDa) |
| Cellular Localization | Mitochondrion membrane>Multi-pass membrane protein. Nucleus inner membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. |
WB
Western blot analysis IFI27 using Jurkat whole cell lysates.
IHC
IFI27 Antibody at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
IHC
IFI27 Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
