WB
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-6: Jurkat, A549, U251, F9, Lncap and Hela cell lysates , Primary antibody: DLAT Antibody at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 5 minutes.WB
ICC/IF
The image is immunofluorescence of Hela cells using DLAT Antibody at dilution 1/300.| Product Name | Anti-DLAT mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:200-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLAT |
|---|---|
| Gene Synonyms | E2 PBC DLTA PDCE2 PDC-E2 |
| Gene Full Name | dihydrolipoamide S-acetyltransferase |
| Gene Summary | This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009] |
| Alternative Names | dihydrolipoamide S-acetyltransferase DLTA PDCE2 PDC-E2 |
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Mitochondrion. |
WB
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-6: Jurkat, A549, U251, F9, Lncap and Hela cell lysates , Primary antibody: DLAT Antibody at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 5 minutes.
WB
ICC/IF
The image is immunofluorescence of Hela cells using DLAT Antibody at dilution 1/300.| Application Notes | WB:1:200-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
