WB
Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with NLRP3 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.ICC/IF
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using NLRP3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using NLRP3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | NLRP3 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NLRP3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 ICC/IF:1:200-1:800 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NLRP3 |
|---|---|
| Gene Synonyms | AII AVP FCU MWS FCAS KEFH CIAS1 FCAS1 NALP3 C1orf7 CLR1.1 DFNA34 PYPAF1 AGTAVPRL |
| Gene Full Name | NLR family pyrin domain containing 3 |
| Gene Summary | This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020] |
| Alternative Names | 3H1A7 Angiotensin/vasopressin receptor AII/AVP-like C1orf7 CIAS1 Cold-induced autoinflammatory syndrome 1 protein |
| Molecular Weight(MW) | 118 kDa(Observed band size:110 kDa) |
| Cellular Localization | Cytoplasm,Cytoskeleton,Endoplasmic reticulum,Golgi apparatus,Inflammasome,Membrane,Mitochondrion,Nucleus ,Secreted |
WB
Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with NLRP3 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.
ICC/IF
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using NLRP3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using NLRP3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:2000-1:10000 ICC/IF:1:200-1:800 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
