WB
HepG2 cells were subjected to SDS PAGE followed by western blot with PAPSS1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
Jurkat cells were subjected to SDS PAGE followed by western blot with PAPSS1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor using PAPSS1 antibody at dilution of 1:100 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor using PAPSS1 antibody at dilution of 1:100 (under 40x lens).IP
IP result of anti-PAPSS1 (4ug; 1:1000) with HepG2 cells lysate 1000ug.ICC/IP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAPSS1 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Product Name | PAPSS1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PAPSS1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 ICC/IF:1:400-1:1600 IHC:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAPSS1 |
|---|---|
| Gene Synonyms | SK1 PAPSS ATPSK1 |
| Gene Full Name | 3'-phosphoadenosine 5'-phosphosulfate synthase 1 |
| Gene Summary | Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008] |
| Alternative Names | 3'-phosphoadenosine-5'-phosphosulfate synthase Adenosine-5'-phosphosulfate 3'-phosphotransferase Adenylylsulfate 3'-phosphotransferase Adenylyl-sulfate kinase APS kinase |
| Molecular Weight(MW) | 71 kDa(Observed band size: 70 kDa) |
| Cellular Localization | cytosol,nucleus |
WB
HepG2 cells were subjected to SDS PAGE followed by western blot with PAPSS1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
Jurkat cells were subjected to SDS PAGE followed by western blot with PAPSS1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor using PAPSS1 antibody at dilution of 1:100 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor using PAPSS1 antibody at dilution of 1:100 (under 40x lens).
IP
IP result of anti-PAPSS1 (4ug; 1:1000) with HepG2 cells lysate 1000ug.
ICC/IP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAPSS1 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:500-1:1000 ICC/IF:1:400-1:1600 IHC:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
