WB
Western blot analysis of nestin expression in TE671 (A) and RD (B) whole cell lysates.IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue shows cytoplasmic and membrane staining of glomerular cells.IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue shows cytoplasmic and membrane staining of myoepithelial cells.| Product Name | Mouse Monoclonal antibody to nestin |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against a 150 amino acid epitope mapping near the C-terminus of nestin of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NES |
|---|---|
| Gene Synonyms | Nbla00170 |
| Gene Full Name | nestin |
| Gene Summary | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| Molecular Weight(MW) | 190 kDa-200 kDa |
| Cellular Localization | Intermediate filament |
WB
Western blot analysis of nestin expression in TE671 (A) and RD (B) whole cell lysates.
IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue shows cytoplasmic and membrane staining of glomerular cells.
IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue shows cytoplasmic and membrane staining of myoepithelial cells.| Application Notes | WB:1:100-1:1000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
