WB
Western Blot analysis of A549 cells using Somatostatin Polyclonal Antibody.WB
Western blot analysis of lysates from A549 cells, using Somatostatin Antibody. The lane on the right is blocked with the synthesized peptide.WB
Western blot analysis of lysates from 1) PC-12, 2) A549 ,3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.IHC
Immunohistochemical analysis of paraffin-embedded Human Gastric fundus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).IHC
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Somatostatin Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | Somatostatin Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Somatostatin. AA range:10-59. |
| Modification | p-Thr982 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SST |
|---|---|
| Gene Synonyms | SMST SST1 |
| Gene Full Name | somatostatin |
| Gene Summary | The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008] |
| Alternative Names | SST Somatostatin Growth hormone release-inhibiting factor |
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Secreted. |
WB
Western Blot analysis of A549 cells using Somatostatin Polyclonal Antibody.
WB
Western blot analysis of lysates from A549 cells, using Somatostatin Antibody. The lane on the right is blocked with the synthesized peptide.
WB
Western blot analysis of lysates from 1) PC-12, 2) A549 ,3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
IHC
Immunohistochemical analysis of paraffin-embedded Human Gastric fundus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
IHC
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Somatostatin Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
