WB
Western blot analysis of Phospho-MYPT1 (T853) on different lysates with Rabbit anti-Phospho-MYPT1 (T853) antibody (HA723023) at 1/2,000 dilution. Lane 1: 293T cell lysate, Lane 2: HeLa cell lysate, Lane 3: HeLa treated with 10μM Y-27632 for 1 hour cell lysate, Lane 4: C2C12 cell lysate, Lane 5: RAW264.7 cell lysate, Lane 6: C6 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr853 of Human MYPT1. |
| Modification | p-T853 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPP1R12A |
|---|---|
| Gene Synonyms | MBS GUBS M130 MYPT1 |
| Gene Full Name | protein phosphatase 1 regulatory subunit 12A |
| Gene Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| Alternative Names | M130 antibody MBS antibody Myosin binding subunit antibody Myosin phosphatase target subunit 1 antibody Myosin phosphatase targeting subunit 1 antibody Myosin phosphatase-targeting subunit 1 antibody MYPT1 antibody MYPT1_HUMAN antibody PPP1R12A antibody Protein phosphatase 1 regulatory inhibitor subunit 12A antibody M130 antibody MBS antibody Myosin binding subunit antibody Myosin phosphatase target subunit 1 antibody Myosin phosphatase targeting subunit 1 antibody Myosin phosphatase-targeting subunit 1 antibody MYPT1 antibody MYPT1_HUMAN antibody PPP1R12A antibody Protein phosphatase 1 regulatory inhibitor subunit 12A antibody Protein phosphatase 1 regulatory subunit 12A antibody Protein phosphatase 1 regulatory (inhibitor) subunit 12A antibody Protein phosphatase myosin binding subunit antibody Protein phosphatase myosin-binding subunit antibody |
| Molecular Weight(MW) | 115 kDa(Observed band size: 140 kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton, stress fiber. |
WB
Western blot analysis of Phospho-MYPT1 (T853) on different lysates with Rabbit anti-Phospho-MYPT1 (T853) antibody (HA723023) at 1/2,000 dilution. Lane 1: 293T cell lysate, Lane 2: HeLa cell lysate, Lane 3: HeLa treated with 10μM Y-27632 for 1 hour cell lysate, Lane 4: C2C12 cell lysate, Lane 5: RAW264.7 cell lysate, Lane 6: C6 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
