WB
Western blot analysis of Pirh2 on different lysates with Rabbit anti-Pirh2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: RAW264.7 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Pirh2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within N-terminal Human Pirh2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RCHY1 |
|---|---|
| Gene Synonyms | ZCHY ARNIP CHIMP PIRH2 RNF199 ZNF363 PRO1996 |
| Gene Full Name | ring finger and CHY zinc finger domain containing 1 |
| Gene Summary | The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013] |
| Alternative Names | Androgen receptor N terminal interacting protein antibody Androgen receptor N-terminal-interacting protein antibody ARNIP antibody CH-rich-interacting match with PLAG1 antibody CHIMP antibody E3 ubiquitin-protein ligase Pirh2 antibody hARNIP antibody hPirh2 antibody p53 induced protein with a RING H2 domain antibody p53-induced RING-H2 protein antibody Androgen receptor N terminal interacting protein antibody Androgen receptor N-terminal-interacting protein antibody ARNIP antibody CH-rich-interacting match with PLAG1 antibody CHIMP antibody E3 ubiquitin-protein ligase Pirh2 antibody hARNIP antibody hPirh2 antibody p53 induced protein with a RING H2 domain antibody p53-induced RING-H2 protein antibody PIRH2E antibody PIRH2F antibody PRO1996 antibody RCHY1 antibody Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase antibody RING finger and CHY zinc finger domain-containing protein 1 antibody RING finger protein 199 antibody RNF199 antibody ZCHY antibody ZFP 363 antibody zinc finger CHY type antibody Zinc finger protein 363 antibody ZN363_HUMAN antibody ZNF363 antibody |
| Molecular Weight(MW) | 30 kDa(Observed band size: 27 kDa) |
| Cellular Localization | Nucleus, Nucleus speckle, Cytoplasm. |
WB
Western blot analysis of Pirh2 on different lysates with Rabbit anti-Pirh2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: RAW264.7 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
