WB
HeLa cells were subjected to SDS PAGE followed by western blot with NIPBL antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.WB
HeLa cells were subjected to SDS PAGE followed by western blot with NIPBL antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using NIPBL antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | NIPBL Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NIPBL protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NIPBL |
|---|---|
| Gene Synonyms | CDLS IDN3 Scc2 CDLS1 IDN3-B |
| Gene Full Name | NIPBL cohesin loading factor |
| Gene Summary | This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and cognitive disability. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | CDLS CDLS1 Delangin IDN3 IDN3 B NIPBL Nipped B homolog (Drosophila) Nipped B like protein SCC2 homolog |
| Molecular Weight(MW) | 316 kDa(Observed band size: 300 kDa) |
| Cellular Localization | Chromosome,Nucleus |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with NIPBL antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB
HeLa cells were subjected to SDS PAGE followed by western blot with NIPBL antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using NIPBL antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
