WB
Western blot analysis of hnRNP K/J expression in MDA-MB-231 (A), NIH/3T3 (B),LADMAC (C), L8 (D) and RPE-J (E) whole cell lysates.IHC
Immunoperoxidase stainingof formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear and cytoplasmic staining of glandular cells.FC
Intracellular FCM analysis was performed on fixed and permeabilized NIH/3T3 cells. The black histogram represents the isotype control, normal mouse IgG2b.| Product Name | Mouse Monoclonal antibody to hnRNP K/J |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against hnRNPs of human origin purified by oligo (dc) affinity chromatography. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | FCMIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 FCM:1 µg per 1 x 106 cells |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNRNPK |
|---|---|
| Gene Synonyms | AUKS CSBP TUNP HNRPK |
| Gene Full Name | heterogeneous nuclear ribonucleoprotein K |
| Gene Summary | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 66/64 kDa |
| Cellular Localization | Cell junction,Cell projection,Cytoplasm,Nucleus,Spliceosome |
WB
Western blot analysis of hnRNP K/J expression in MDA-MB-231 (A), NIH/3T3 (B),LADMAC (C), L8 (D) and RPE-J (E) whole cell lysates.
IHC
Immunoperoxidase stainingof formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear and cytoplasmic staining of glandular cells.
FC
Intracellular FCM analysis was performed on fixed and permeabilized NIH/3T3 cells. The black histogram represents the isotype control, normal mouse IgG2b.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 FCM:1 µg per 1 x 106 cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
