WB
Western blot analysis of aggrecan expression in human articular cartilage predigested with chondroitinase ABC, keratanase I and endo-β-galactosidase.IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cartilage tissue shows cytoplasmic staining in chondrocytes.IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human soft tissue shows cytoplasmic staining of chondrocytes under low (A) and high (B) magnification.| Product Name | Mouse Monoclonal antibody to aggrecan |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against human articular cartilage aggrecan. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACAN |
|---|---|
| Gene Synonyms | AGC1 SEDK AGCAN CSPG1 MSK16 CSPGCP SSOAOD |
| Gene Full Name | aggrecan |
| Gene Summary | This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 200 kDa |
| Cellular Localization | Extracellular matrix,Secreted |
WB
Western blot analysis of aggrecan expression in human articular cartilage predigested with chondroitinase ABC, keratanase I and endo-β-galactosidase.
IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cartilage tissue shows cytoplasmic staining in chondrocytes.
IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human soft tissue shows cytoplasmic staining of chondrocytes under low (A) and high (B) magnification.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
