WB
Western blot analysis of extracts from various samples, using VCPIP1 Antibody at 1/1000 dilution. Lane 1: Colo205 cells(heat-shock treatment). Lane 2: Rat brain.IHC
VCPIP1 Antibody at 1/100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.ICC/IF
VCPIP1 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to VCPIP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human VCPIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCPIP1 |
|---|---|
| Gene Synonyms | DUBA3 VCIP135 |
| Gene Full Name | valosin containing protein interacting protein 1 |
| Gene Summary | Enables cysteine-type deubiquitinase activity. Involved in protein deubiquitination; protein-DNA covalent cross-linking repair; and regulation of protein localization to chromatin. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Deubiquitinating protein VCIP135 DUBA3 KIAA1850 Valosin containing protein (p97)/p47 complex interacting protein 1 Valosin containing protein p97/p47 complex interacting protein 1 Valosin containing protein p97/p47 complex interacting protein p135 Valosin-containing protein p97/p47 complex-interacting protein 1 Valosin-containing protein p97/p47 complex-interacting protein p135 VCIP1_HUMAN VCIP135 VCP/p47 complex-interacting 135-kDa protein Vcpip1 |
| Molecular Weight(MW) | 134 kDa(Observed band size: 150 kD) |
| Cellular Localization | Endoplasmic reticulum. Golgi apparatus>Golgi stack. |
WB
Western blot analysis of extracts from various samples, using VCPIP1 Antibody at 1/1000 dilution. Lane 1: Colo205 cells(heat-shock treatment). Lane 2: Rat brain.
IHC
VCPIP1 Antibody at 1/100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ICC/IF
VCPIP1 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
