WB
Western blot analysis of RPE65 expression in mouse eye tissue extract.| Product Name | Mouse Monoclonal antibody to RPE65 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against RPE microsomal membranes of bovine origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPE65 |
|---|---|
| Gene Synonyms | p63 BCO3 LCA2 RP20 rd12 mRPE65 sRPE65 |
| Gene Full Name | retinoid isomerohydrolase RPE65 |
| Gene Summary | The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017] |
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Endoplasmic reticulum,Membrane,Microsome |
WB
Western blot analysis of RPE65 expression in mouse eye tissue extract.| Application Notes | WB:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
