WB
Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using MTDH mouse mAb with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using MTDH mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.FC
Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to MTDH |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human MTDH (AA: 200-450) expressed in mammalian. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:50-1:200 FC:1:200-1:400 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTDH |
|---|---|
| Gene Synonyms | 3D3 AEG1 AEG-1 LYRIC LYRIC/3D3 |
| Gene Full Name | metadherin |
| Gene Summary | Enables NF-kappaB binding activity; double-stranded RNA binding activity; and transcription coactivator activity. Involved in several processes, including lipopolysaccharide-mediated signaling pathway; positive regulation of intracellular signal transduction; and regulation of DNA-templated transcription. Located in endoplasmic reticulum; nuclear lumen; and perinuclear region of cytoplasm. Implicated in hepatocellular carcinoma. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 3D3 AEG1 AEG-1 LYRIC LYRIC/3D3 |
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Cell junction,Cytoplasm,Endoplasmic reticulum,Membrane,Nucleus,Tight junction |
WB
Western blot analysis using MTDH mouse mAb against MCF-7 (1), T47D (2), Jurkat (3) ,K562 (4), Hela(5), PC-3(6), HepG2(7), A431(8) and COS7 (9) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using MTDH mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using MTDH mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using MTDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.
FC
Flow cytometric analysis of Hela cells using MTDH mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:50-1:200 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
