WB
Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-ERCC3 monoclonal antibody (1:500).IHC
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Product Name | XPB (ERCC3) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 191-436 of human ERCC3 (NP_000113) produced in E.coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500 IHC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | ERCC3 |
|---|---|
| Gene Synonyms | XPB BTF2 Ssl2 TTD2 GTF2H RAD25 TFIIH |
| Gene Full Name | ERCC excision repair 3, TFIIH core complex helicase subunit |
| Gene Summary | This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | BTF2 GTF2H RAD25 Ssl2 TFIIH TTD2 XPB |
| Molecular Weight(MW) | 89.1 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-ERCC3 monoclonal antibody (1:500).
IHC
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Application Notes | WB:1:500 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
