WB
Western blot analysis of extracts from various samples, using TAK1 Mouse Monoclonal Antibody. Lane 1: Mouse spleen, blocked with antigen-specific peptides. Lane 2: Mouse spleen. Lane 3: A549 cells. Lane 4: Mcf7 cells.WB
Western blot analysis of extracts from various samples, using TAK1 Mouse Monoclonal Antibody. Lane 1: Hela cells, blocked with antigen-specific peptides. Lane 2: Hela cells. Lane 3: Huvec cells. Lane 4: Hepg2 cells.IHC
TAK1 Mouse Monoclonal Antibody at 1/100 staining mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.IHC
TAK1 Mouse Monoclonal Antibody at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.| Product Name | Mouse monoclonal antibody to TAK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human TAK1, corresponding to a region within the internal amino acids. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:3000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K7 |
|---|---|
| Gene Synonyms | CSCF FMD2 TAK1 MEKK7 TGF1a |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 7 |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | M3K7_HUMAN MAP3K 7 Map3k7 MEKK7 Mitogen activated protein kinase kinase kinase 7 Mitogen-activated protein kinase kinase kinase 7 TAK1 TGF beta activated kinase 1 TGF-beta-activated kinase 1 TGF1a Transforming growth factor beta activated kinase 1 Transforming growth factor-beta-activated kinase 1 |
| Molecular Weight(MW) | 67 kDa(Observed band size: 70 kDa) |
| Cellular Localization | Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. |
WB
Western blot analysis of extracts from various samples, using TAK1 Mouse Monoclonal Antibody. Lane 1: Mouse spleen, blocked with antigen-specific peptides. Lane 2: Mouse spleen. Lane 3: A549 cells. Lane 4: Mcf7 cells.
WB
Western blot analysis of extracts from various samples, using TAK1 Mouse Monoclonal Antibody. Lane 1: Hela cells, blocked with antigen-specific peptides. Lane 2: Hela cells. Lane 3: Huvec cells. Lane 4: Hepg2 cells.
IHC
TAK1 Mouse Monoclonal Antibody at 1/100 staining mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.
IHC
TAK1 Mouse Monoclonal Antibody at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
