WB
Western blot analysis of CCR9 using HeLa whole cell lysates.ICC/IF
CCR9 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to CCR9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human CCR9, corresponding to a region within C-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:3000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCR9 |
|---|---|
| Gene Synonyms | GPR28 CDw199 GPR-9-6 CC-CKR-9 |
| Gene Full Name | C-C motif chemokine receptor 9 |
| Gene Summary | The protein encoded by this gene is a G protein-coupled receptor with seven transmembrane domains that belongs to the beta chemokine receptor family. Chemokines and their receptors are key regulators of thymocyte migration and maturation in normal and inflammation conditions. This gene is differentially expressed in T lymphocytes of the small intestine and colon, and its interaction with chemokine 25 contributes to intestinal intra-epithelial lymphocyte homing to the small intestine. This suggests a role for this gene in directing immune responses to different segments of the gastrointestinal tract. This gene and its exclusive ligand, chemokine 25, are overexpressed in a variety of malignant tumors and are closely associated with tumor proliferation, apoptosis, invasion, migration and drug resistance. This gene maps to the chemokine receptor gene cluster. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] |
| Alternative Names | C C chemokine receptor D6 C C chemokine receptor type 9 C C CKR 9 CC Chemokine receptor CCR10 CC CKR 9 CCBP2 CCCKR9 CCR 9 CCR9 CDw199 CDw199 antigen Chemokine (C C motif) receptor 9 Chemokine (C C motif) receptor 9 isoform CRA_a Chemokine (C-C motif) receptor 9 isoform A Chemokine (C-C motif) receptor 9 isoform B Chemokine C C motif receptor 9 Chemokine C C receptor 10 Chemokine CC Motif Receptor 9 Chemokine receptor CCR 9 Chemokine receptor CCR9 Chemokine-binding protein D6 Cmkbr10 CMKBR9 G protein coupled receptor 28 GPR 28 GPR 9 6 GPR28 GPR96 OTTHUMP00000164653 OTTHUMP00000164654 |
| Molecular Weight(MW) | 42 kDa(Observed band size: 45 kDa) |
| Cellular Localization | Cell membrane>Multi-pass membrane protein. |
WB
Western blot analysis of CCR9 using HeLa whole cell lysates.
ICC/IF
CCR9 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:1000-1:3000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
