WB
Western blot analysis of FEZF1 using anti-FEZF1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FEZF1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of FEZF1 using anti-FEZF1 antibody. FEZF1 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-FEZF1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green)was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U2OS cells using anti-FEZF1 antibody. Overlay histogram showing U2OS cells stained with anti-FEZF1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FEZF1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to FEZF1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human FEZF1 recombinant protein (Position: A45-K258). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCMICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FCM:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FEZF1 |
|---|---|
| Gene Synonyms | FEZ HH22 ZNF312B |
| Gene Full Name | FEZ family zinc finger 1 |
| Gene Summary | This gene encodes a transcriptional repressor that belongs to the zinc finger double domain protein family. The encoded protein is thought to play a role in the embryonic migration of gonadotropin-releasing hormone neurons into the brain. Mutations in this gene are associated with hypogonadotropic hypogonadism-22 with anosmia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| Alternative Names | Fez family zinc finger protein 1 Zinc finger protein 312B FEZF1 FEZ ZNF312B |
| Molecular Weight(MW) | 52 kDa(Observed band size: 65 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of FEZF1 using anti-FEZF1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FEZF1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of FEZF1 using anti-FEZF1 antibody. FEZF1 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-FEZF1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green)was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U2OS cells using anti-FEZF1 antibody. Overlay histogram showing U2OS cells stained with anti-FEZF1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FEZF1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FCM:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
