WB
Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody at 1/1,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane), Lane 2: K-562 cell lysate (10 µg/Lane), Lane 3: Jurkat cell lysate (10 µg/Lane), Lane 4: MCF7 cell lysate (10 µg/Lane), Lane 5: HeLa cell lysate (10 µg/Lane), Lane 6: SK-Br-3 cell lysate (10 µg/Lane), Lane 7: SW480 cell lysate (10 µg/Lane), Lane 8: THP-1 cell lysate (10 µg/Lane), Lane 9: A549 cell lysate (10 µg/Lane), Lane 10: HepG2 cell lysate (10 µg/Lane), Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | FADD Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human FADD aa 1-170 / 208. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:200 ICC/IF:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FADD |
|---|---|
| Gene Synonyms | GIG3 IMD90 MORT1 |
| Gene Full Name | Fas associated via death domain |
| Gene Summary | The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008] |
| Alternative Names | FADD antibody FADD protein antibody FADD_HUMAN antibody Fas (TNFRSF6) associated via death domain antibody Fas associated via death domain antibody Fas associating death domain containing protein antibody Fas associating protein antibody Fas associating protein with death domain antibody Fas TNFRSF6 associated via death domain antibody FAS-associated death domain protein antibody FADD antibody FADD protein antibody FADD_HUMAN antibody Fas (TNFRSF6) associated via death domain antibody Fas associated via death domain antibody Fas associating death domain containing protein antibody Fas associating protein antibody Fas associating protein with death domain antibody Fas TNFRSF6 associated via death domain antibody FAS-associated death domain protein antibody FAS-associating death domain-containing protein antibody GIG 3 antibody GIG3 antibody Growth inhibiting gene 3 protein antibody Growth-inhibiting gene 3 protein antibody H sapiens mRNA for mediator of receptor induced toxicity antibody Mediator of receptor induced toxicity antibody MGC8528 antibody MORT 1 antibody MORT1 antibody Protein FADD antibody |
| Molecular Weight(MW) | 23 kDa(Observed band size: 25 kDa) |
| Cellular Localization | Cytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome. |
WB
Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody at 1/1,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane), Lane 2: K-562 cell lysate (10 µg/Lane), Lane 3: Jurkat cell lysate (10 µg/Lane), Lane 4: MCF7 cell lysate (10 µg/Lane), Lane 5: HeLa cell lysate (10 µg/Lane), Lane 6: SK-Br-3 cell lysate (10 µg/Lane), Lane 7: SW480 cell lysate (10 µg/Lane), Lane 8: THP-1 cell lysate (10 µg/Lane), Lane 9: A549 cell lysate (10 µg/Lane), Lane 10: HepG2 cell lysate (10 µg/Lane), Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:200 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
