WB
Direct Western blot analysis of LDH-A expression in whole cell lysates of HeLa (A), Y79 (B), SJRH30 (C), SK-N-SH (D), A-431 (E) and Hs 181 Tes (F).IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human small intestine tissue showed cytoplasmic staining of glandular cells.ICC/IF
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Mouse Monoclonal antibody to LDH-A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | amino acids 6-42 at the N-terminus of LDH-A of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDHA |
|---|---|
| Gene Synonyms | LDHM GSD11 PIG19 HEL-S-133P |
| Gene Full Name | lactate dehydrogenase A |
| Gene Summary | This gene encodes the A subunit of lactate dehydrogenase enzyme which catalyzes the reversible conversion of pyruvate to lactate with the concomitant oxidation of NADH to NAD in anaerobic glycolysis. The protein is found predominantly in skeletal muscle and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2023] |
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cytoplasm |
WB
Direct Western blot analysis of LDH-A expression in whole cell lysates of HeLa (A), Y79 (B), SJRH30 (C), SK-N-SH (D), A-431 (E) and Hs 181 Tes (F).
IHC
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human small intestine tissue showed cytoplasmic staining of glandular cells.
ICC/IF
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
