IHC
Phospho-Gli3 (Ser664) Antibody at 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.IHC
Phospho-Gli3 (Ser664) Antibody at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Phospho-Gli3 (Ser664) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Gli3 around the phosphorylation site of Ser664. |
| Modification | p-Ser664 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLI3 |
|---|---|
| Gene Synonyms | PHS ACLS GCPS PAPA PAPB PAP-A PAPA1 PPDIV GLI3FL GLI3-190 |
| Gene Full Name | GLI family zinc finger 3 |
| Gene Summary | This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008] |
| Alternative Names | ACLS DNA binding protein GCPS Gli 3 GLI family zinc finger 3 GLI Kruppel family member GLI 3 GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) GLI Kruppel family member GLI3 GLI3 GLI3 C-terminally truncated form GLI3 form of 190 kDa GLI3 form of 83 kDa GLI3 full length protein GLI3-190 GLI3-83 GLI3_HUMAN GLI3FL Glioma associated oncogene family zinc finger 3 Oncogene GLI3 PAP A PAPA 1 PAPA PAPA1 PAPB PHS PPD IV PPDIV Transcriptional activator GLI3 Transcriptional repressor GLI3R Zinc finger protein GLI 3 Zinc finger protein GLI3 |
| Molecular Weight(MW) | 170 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Cell projection>Cilium. |
IHC
Phospho-Gli3 (Ser664) Antibody at 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
IHC
Phospho-Gli3 (Ser664) Antibody at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
