WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ULK1 (Phospho Ser757) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 was treated with EGF ( 100ng/mL) for 20 minutes Predicted band size: 113kDa Observed band size: 150kDa Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ULK1 (Phospho Ser757) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Product Name | ULK1 (Phospho Ser757) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Ser757 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ULK1 |
|---|---|
| Gene Synonyms | ATG1 ATG1A UNC51 hATG1 Unc51.1 |
| Gene Full Name | unc-51 like autophagy activating kinase 1 |
| Gene Summary | Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; protein phosphorylation; and regulation of autophagy. Located in bounding membrane of organelle; cytosol; and phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex. Is active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ULK1 KIAA0722 Serine/threonine-protein kinase ULK1 Autophagy-related protein 1 homolog ATG1 hATG1 Unc-51-like kinase 1 |
| Molecular Weight(MW) | 113 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Cytoplasm , cytosol . Preautophagosomal structure . Under starvation conditions , is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ULK1 (Phospho Ser757) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 was treated with EGF ( 100ng/mL) for 20 minutes Predicted band size: 113kDa Observed band size: 150kDa Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ULK1 (Phospho Ser757) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
