WB
Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PSAP antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: LnCapIHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-Prostatic Acid Phosphatase antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-Prostatic Acid Phosphatase antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Mouse anti-Prostatic Acid Phosphatase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Prostatic Acid Phosphatase Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human PSAP AA range: 201-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACP3 |
|---|---|
| Gene Synonyms | ACPP 5'-NT ACP-3 TM-PAP |
| Gene Full Name | acid phosphatase 3 |
| Gene Summary | This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008] |
| Alternative Names | 5' NT antibody 5' nucleotidase antibody 5'-NT antibody 5'-nucleotidase antibody Acid phosphatase prostate antibody ACP 3 antibody ACP3 antibody acpP antibody Ecto 5' nucleotidase antibody Ecto-5'-nucleotidase antibody 5' NT antibody 5' nucleotidase antibody 5'-NT antibody 5'-nucleotidase antibody Acid phosphatase prostate antibody ACP 3 antibody ACP3 antibody acpP antibody Ecto 5' nucleotidase antibody Ecto-5'-nucleotidase antibody PAP antibody PPAP_HUMAN antibody Prostatic acid phosphatase antibody Prostatic acid phosphotase antibody Thiamine monophosphatase antibody TMPase antibody |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Secreted, Lysosome, Cell membrane. |
WB
Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PSAP antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: LnCap
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-Prostatic Acid Phosphatase antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-Prostatic Acid Phosphatase antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Mouse anti-Prostatic Acid Phosphatase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IHC:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.. |
