WB
Western blot analysis of TOPBP1 using anti-TOPBP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TOPBP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TOPBP1 using anti-TOPBP1 antibody TOPBP1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-TOPBP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TOPBP1 using anti-TOPBP1 antibody TOPBP1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-TOPBP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of TOPBP1 using anti-TOPBP1 antibody and anti-Beta Tubulin antibody. TOPBP1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TOPBP1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IP
IP analysis of TOPBP1 using anti-TOPBP1 antibody in Hela whole cell lysate. Western blot analysis of TOPBP1 using anti- TOPBP1 antibody. Lane 1: Hela whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- TOPBP1 antibody in Hela whole cell lysate, Lane 3: anti- TOPBP1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- TOPBP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to TOPBP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human TOPBP1 recombinant protein (Position: E217-Q1459). Human TOPBP1 shares 75.3% amino acid (aa) sequence identity with mouse TOPBP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOPBP1 |
|---|---|
| Gene Synonyms | Dpb11 TOP2BP1 |
| Gene Full Name | DNA topoisomerase II binding protein 1 |
| Gene Summary | This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008] |
| Alternative Names | KIAA0259 TOP2BP1 TOPBP1 |
| Molecular Weight(MW) | 170 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Cytoskeleton,Nucleus |
WB
Western blot analysis of TOPBP1 using anti-TOPBP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TOPBP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TOPBP1 using anti-TOPBP1 antibody TOPBP1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-TOPBP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TOPBP1 using anti-TOPBP1 antibody TOPBP1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-TOPBP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of TOPBP1 using anti-TOPBP1 antibody and anti-Beta Tubulin antibody. TOPBP1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TOPBP1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IP
IP analysis of TOPBP1 using anti-TOPBP1 antibody in Hela whole cell lysate. Western blot analysis of TOPBP1 using anti- TOPBP1 antibody. Lane 1: Hela whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- TOPBP1 antibody in Hela whole cell lysate, Lane 3: anti- TOPBP1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- TOPBP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
