WB
Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 1 minute 7 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Ryanodine receptor 2 / RYR-2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human RYR-2 aa 1,301-1,600. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Ryr2 |
|---|---|
| Gene Synonyms | RYR-2 9330127I20Rik |
| Gene Full Name | ryanodine receptor 2, cardiac |
| Gene Summary | Enables several functions, including calmodulin binding activity; identical protein binding activity; and ryanodine-sensitive calcium-release channel activity. Involved in several processes, including cellular response to caffeine; cellular response to epinephrine stimulus; and regulation of heart contraction. Acts upstream of or within several processes, including cardiac muscle hypertrophy; left ventricular cardiac muscle tissue morphogenesis; and positive regulation of heart rate. Located in Z disc; sarcoplasmic reticulum membrane; and smooth endoplasmic reticulum. Part of calcium channel complex. Is expressed in several structures, including brain; cardiovascular system; genitourinary system; gut; and respiratory system. Used to study catecholaminergic polymorphic ventricular tachycardia 1 and heart disease. Human ortholog(s) of this gene implicated in arrhythmogenic right ventricular cardiomyopathy and catecholaminergic polymorphic ventricular tachycardia 1. Orthologous to human RYR2 (ryanodine receptor 2). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ARVC2 antibody ARVD2 antibody Cardiac muscle ryanodine receptor antibody Cardiac muscle ryanodine receptor-calcium release channel antibody hRYR-2 antibody ryanodine receptor 2 (cardiac) antibody Ryanodine receptor 2 antibody RyR antibody RYR-2 antibody RyR2 antibody ARVC2 antibody ARVD2 antibody Cardiac muscle ryanodine receptor antibody Cardiac muscle ryanodine receptor-calcium release channel antibody hRYR-2 antibody ryanodine receptor 2 (cardiac) antibody Ryanodine receptor 2 antibody RyR antibody RYR-2 antibody RyR2 antibody RYR2_HUMAN antibody Type 2 ryanodine receptor antibody VTSIP antibody |
| Molecular Weight(MW) | 565 kDa |
| Cellular Localization | Sarcoplasmic reticulum membrane. |
WB
Western blot analysis of Ryanodine receptor 2 / RYR-2 on different lysates with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 1 minute 7 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
