WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FURIN antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human placenta was stained with anti-FURIN Rabbit antibody.IHC
Mouse kidney was stained with anti-FURIN Rabbit antibody.| Product Name | FURIN Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FURIN |
|---|---|
| Gene Synonyms | FUR PACE SPC1 PCSK3 |
| Gene Full Name | furin, paired basic amino acid cleaving enzyme |
| Gene Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020] |
| Molecular Weight(MW) | 87 kDa |
| Cellular Localization | Golgi apparatus , trans-Golgi network membrane ; Single-pass type I membrane protein . Cell membrane ; Single-pass type I membrane protein . Secreted . Endosome membrane ; Single-pass type I membrane protein . Shuttles between the trans-Golgi network and the cell surface (PubMed:9412467 , PubMed:11799113) . Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER) . A second cleavage within the propeptide occurs in the trans Golgi network (TGN) , followed by the release of the propeptide and the activation of furin (PubMed:11799113). |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FURIN antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human placenta was stained with anti-FURIN Rabbit antibody.
IHC
Mouse kidney was stained with anti-FURIN Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
