WB
50 ng Recombinant M.tuberculosis Ag85B/fbpB protein, N-His per lane probed with M.tuberculosis Ag85B/fbpB polyclonal antibody respectively, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.| Product Name | M.tuberculosis Ag85B/fbpB Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant M.tuberculosis Ag85B/fbpB protein : 41-325/325 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ag85B |
|---|---|
| Gene Synonyms | fbpB |
| Target | M.tuberculosis (predicted: MYCTU) |
| Alternative Names | A85B_MYCTU Diacylglycerol acyltransferase/mycolyltransferase Ag85B DGAT 30 kDa extracellular protein Acyl-CoA:diacylglycerol acyltransferase Antigen 85 complex B 85B Extracellular alpha-antigen Fibronectin-binding protein B Fbps B MTCY180.32 Rv1886c |
| Molecular Weight(MW) | 35 kDa |
| Function | The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. |
| Cellular Localization | Secreted |
WB
50 ng Recombinant M.tuberculosis Ag85B/fbpB protein, N-His per lane probed with M.tuberculosis Ag85B/fbpB polyclonal antibody respectively, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
