WB
Western blot analysis of ALADIN/AAAS using anti-ALADIN/AAAS antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALADIN/AAAS antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of U2OS cells using anti-ALADIN/AAAS antibody.Overlay histogram showing U2OS cells stained with anti-ALADIN/AAAS antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALADIN/AAAS Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ALADIN/AAAS |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ALADIN/AAAS recombinant protein (Position: R13-K466). Human ALADIN/AAAS shares 94.3% amino acid (aa) sequence identity with mouse ALADIN/AAAS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AAAS |
|---|---|
| Gene Synonyms | AAA AAASb GL003 ALADIN ADRACALA ADRACALIN |
| Gene Full Name | aladin WD repeat nucleoporin |
| Gene Summary | The protein encoded by this gene is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. The encoded protein is part of the nuclear pore complex and is anchored there by NDC1. Defects in this gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| Alternative Names | AAA AAAS AAASb ADRACALA ADRACALIN ALADIN GL003 |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Nuclear pore complex,Nucleus |
WB
Western blot analysis of ALADIN/AAAS using anti-ALADIN/AAAS antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALADIN/AAAS antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of U2OS cells using anti-ALADIN/AAAS antibody.Overlay histogram showing U2OS cells stained with anti-ALADIN/AAAS antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALADIN/AAAS Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
