WB
Western blot analysis of Laminin gamma 1 on different lysates with Rabbit anti-Laminin gamma 1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: OVCAR-3 cell lysate, Lane 4: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | Laminin gamma 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Laminin gamma 1 aa 21-70 / 1,609. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LAMC1 |
|---|---|
| Gene Synonyms | LAMB2 |
| Gene Full Name | laminin subunit gamma 1 |
| Gene Summary | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011] |
| Alternative Names | LAMB2 antibody lamc1 antibody LAMC1_HUMAN antibody Laminin B2 chain antibody Laminin subunit gamma-1 antibody Laminin gamma 1 (formerly LAMB2) antibody Laminin-1 subunit gamma antibody Laminin-10 subunit gamma antibody Laminin-11 subunit gamma antibody Laminin-2 subunit gamma antibody LAMB2 antibody lamc1 antibody LAMC1_HUMAN antibody Laminin B2 chain antibody Laminin subunit gamma-1 antibody Laminin gamma 1 (formerly LAMB2) antibody Laminin-1 subunit gamma antibody Laminin-10 subunit gamma antibody Laminin-11 subunit gamma antibody Laminin-2 subunit gamma antibody Laminin-3 subunit gamma antibody Laminin-4 subunit gamma antibody Laminin-6 subunit gamma antibody Laminin-7 subunit gamma antibody Laminin-8 subunit gamma antibody Laminin-9 subunit gamma antibody MGC87297 antibody S-LAM gamma antibody S-laminin subunit gamma antibody |
| Molecular Weight(MW) | 178 kDa(Observed band size: 250 kDa) |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
WB
Western blot analysis of Laminin gamma 1 on different lysates with Rabbit anti-Laminin gamma 1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: OVCAR-3 cell lysate, Lane 4: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
