WB
Western blot analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody. LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody. LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody.LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis using anti- Laminin antibody.detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondarand counterstained with DAPI (blue).y Antibody (green)IF-P
IF analysis using anti- Laminin antibody.detected in paraffin-embedded section of rat skeletal muscle tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI(blue).| Product Name | Rabbit polyclonal antibody to LAMC1/LAMC2/LAMC3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide mixture of laminin gamma1,2,3(NKLNEIEGSLNKAKDEMKAS; DLEERVRRQRNHLHLLETSI; LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:5000 IF-P:1:50-1:400 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LAMC1 |
|---|---|
| Gene Synonyms | LAMB2 |
| Gene Full Name | laminin subunit gamma 1 |
| Gene Summary | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011] |
| Alternative Names | Lamc1 Lamb-2 Lamc-1 Lamc2 Lamc3 Lamc1 Lamc2 Lamc3 |
| Molecular Weight(MW) | 177 kDa(Observed band size: 220-250 kDa) |
| Cellular Localization | Basement membrane,Extracellular matrix,Secreted |
WB
Western blot analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody. LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody. LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody.LAMC1/LAMC2/LAMC3 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis using anti- Laminin antibody.detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondarand counterstained with DAPI (blue).y Antibody (green)
IF-P
IF analysis using anti- Laminin antibody.detected in paraffin-embedded section of rat skeletal muscle tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI(blue).| Application Notes | WB:1:1000-1:5000 IF-P:1:50-1:400 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
