WB
Western blot analysis of SSBP1 on different lysates with Rabbit anti-SSBP1 antibody at 1/5,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: A375 cell lysate (20 µg/Lane), Lane 3: NIH:OVCAR-3 cell lysate (20 µg/Lane), Lane 4: 293T cell lysate (20 µg/Lane), Lane 5: A431 cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: COS-1 cell lysate (20 µg/Lane), Lane 8: NIH/3T3 cell lysate (20 µg/Lane), Lane 9: Neuro-2a cell lysate (20 µg/Lane), Lane 10: C6 cell lysate (20 µg/Lane), Lane 11: Mouse liver tissue lysate (40 µg/Lane), Lane 12: Mouse pancreas tissue lysate (40 µg/Lane), Lane 13: Rat liver tissue lysate (40 µg/Lane), Lane 14: Rat pancreas tissue lysate (40 µg/Lane), Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SSBP1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SSBP1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SSBP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-SSBP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SSBP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SSBP1 aa 1-148. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SSBP1 |
|---|---|
| Gene Synonyms | SSBP OPA13 mtSSB Mt-SSB SOSS-B1 |
| Gene Full Name | single stranded DNA binding protein 1 |
| Gene Summary | SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al., 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010] |
| Alternative Names | Single-stranded DNA-binding protein mitochondrial antibody Mt SSB antibody Mt-SSB antibody MtSSB antibody PWP1 interacting protein 17 antibody PWP1-interacting protein 17 antibody SENSOR OF SINGLE-STRANDED DNA COMPLEX antibody Single stranded DNA binding protein 1 antibody Single stranded DNA binding protein antibody Single stranded DNA binding protein mitochondrial antibody Single-stranded DNA-binding protein mitochondrial antibody Mt SSB antibody Mt-SSB antibody MtSSB antibody PWP1 interacting protein 17 antibody PWP1-interacting protein 17 antibody SENSOR OF SINGLE-STRANDED DNA COMPLEX antibody Single stranded DNA binding protein 1 antibody Single stranded DNA binding protein antibody Single stranded DNA binding protein mitochondrial antibody Single-stranded DNA-binding protein antibody SOSS B1 antibody SOSS COMPLEX SUBUNIT B1 antibody SSBP 1 antibody SSBP antibody SSBP_HUMAN antibody SSBP1 antibody |
| Molecular Weight(MW) | 17 kDa(Observed band size: 14 kDa) |
| Cellular Localization | Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid. |
WB
Western blot analysis of SSBP1 on different lysates with Rabbit anti-SSBP1 antibody at 1/5,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: A375 cell lysate (20 µg/Lane), Lane 3: NIH:OVCAR-3 cell lysate (20 µg/Lane), Lane 4: 293T cell lysate (20 µg/Lane), Lane 5: A431 cell lysate (20 µg/Lane), Lane 6: MCF7 cell lysate (20 µg/Lane), Lane 7: COS-1 cell lysate (20 µg/Lane), Lane 8: NIH/3T3 cell lysate (20 µg/Lane), Lane 9: Neuro-2a cell lysate (20 µg/Lane), Lane 10: C6 cell lysate (20 µg/Lane), Lane 11: Mouse liver tissue lysate (40 µg/Lane), Lane 12: Mouse pancreas tissue lysate (40 µg/Lane), Lane 13: Rat liver tissue lysate (40 µg/Lane), Lane 14: Rat pancreas tissue lysate (40 µg/Lane), Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SSBP1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SSBP1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SSBP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-SSBP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
