WB
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody at 1/100,000 dilution. Lane 1: Mouse kidney tissue lysate, Lane 2: Mouse thyroid tissue lysate, Lane 3: Mouse lung tissue lysate, Lane 4: Mouse brain tissue lysate, Lane 5: Mouse heart tissue lysate, Lane 6: Mouse ovary tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 30 seconds; Low expression : Mouse brain (PMID: 12000719). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/100,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Species: Mouse, Tissue: Kidney, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/400, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Product Name | Endomucin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse Endomucin aa 1-190. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:100000 IHC:1:400 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Emcn |
|---|---|
| Gene Synonyms | Muc14 0610012K22Rik |
| Gene Full Name | endomucin |
| Gene Summary | Predicted to enable carbohydrate binding activity. Acts upstream of or within hematopoietic stem cell homeostasis. Predicted to be located in plasma membrane. Is expressed in several structures, including cardiovascular system; early conceptus; hemolymphoid system; lung; and urinary system. Orthologous to human EMCN (endomucin). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | EMCN antibody EMCN2 antibody Endomucin 2 antibody Endomucin antibody Endomucin-2 antibody Endothelial sialomucin antibody Gastric cancer antigen Ga34 antibody MUC-14 antibody MUC14 antibody MUCEN_HUMAN antibody EMCN antibody EMCN2 antibody Endomucin 2 antibody Endomucin antibody Endomucin-2 antibody Endothelial sialomucin antibody Gastric cancer antigen Ga34 antibody MUC-14 antibody MUC14 antibody MUCEN_HUMAN antibody Mucin 14 antibody Mucin-14 antibody |
| Molecular Weight(MW) | 28 kDa(Observed band size: 70-100 kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody at 1/100,000 dilution. Lane 1: Mouse kidney tissue lysate, Lane 2: Mouse thyroid tissue lysate, Lane 3: Mouse lung tissue lysate, Lane 4: Mouse brain tissue lysate, Lane 5: Mouse heart tissue lysate, Lane 6: Mouse ovary tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 30 seconds; Low expression : Mouse brain (PMID: 12000719). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/100,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Species: Mouse, Tissue: Kidney, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/400, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Application Notes | WB:1:100000 IHC:1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
