| Product Name | Anti-Staphylococcus aureus Sortase A/SrtA Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli - derived recombinant Staphylococcus aureus Sortase A/SrtA (Gln60-Lys206). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | Staphylococcus aureus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | EC:3.4.22.70, Sortase A, Surface protein sorting A, srtA |
|---|---|
| Function | Sortase A (SrtA) is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction. |
| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Storage Buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
