WB
Western blot analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XLF/NHEJ1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody.XLF/NHEJ1 was detected in a paraffin-embedded section of human skin cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody.XLF/NHEJ1 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody. XLF/NHEJ1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).ICC/IF
ICC/IF analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody. XLF/NHEJ1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Hepa1-6 cells using anti-XLF/NHEJ1 antibody. Overlay histogram showing Hepa1-6 cells stained with anti-XLF/NHEJ1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XLF/NHEJ1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.FC
Flow Cytometry analysis of K562 cells using anti-XLF/NHEJ1 antibody. Overlay histogram showing K562 cells stained with anti-XLF/NHEJ1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XLF/NHEJ1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to XLF/NHEJ1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human XLF/NHEJ1 recombinant protein (Position: M1-E273). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NHEJ1 |
|---|---|
| Gene Synonyms | XLF IMD124 MCOPCB13 |
| Gene Full Name | non-homologous end joining factor 1 |
| Gene Summary | Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008] |
| Alternative Names | NHEJ1 Protein cernunnos XLF XRCC4 like factor |
| Molecular Weight(MW) | 33 kDa(Observed band size: 38 kDa) |
| Cellular Localization | Chromosome,Nucleus |
WB
Western blot analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XLF/NHEJ1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody.XLF/NHEJ1 was detected in a paraffin-embedded section of human skin cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody.XLF/NHEJ1 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody. XLF/NHEJ1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
ICC/IF
ICC/IF analysis of XLF/NHEJ1 using anti-XLF/NHEJ1 antibody. XLF/NHEJ1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-XLF/NHEJ1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Hepa1-6 cells using anti-XLF/NHEJ1 antibody. Overlay histogram showing Hepa1-6 cells stained with anti-XLF/NHEJ1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XLF/NHEJ1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
FC
Flow Cytometry analysis of K562 cells using anti-XLF/NHEJ1 antibody. Overlay histogram showing K562 cells stained with anti-XLF/NHEJ1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XLF/NHEJ1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
