WB
Western blot analysis of anti-Islet 1/ISL1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Islet 1/ISL1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate .IHC
IHC analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody . Islet 1/ISL1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody. Islet 1/ISL1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody. Islet 1/ISL1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.IF-P
IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody. Islet 1/ISL1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)was used as secondary antibody.ICC/IF
ICC/IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody and anti-Beta Tubulin antibody.Islet 1/ISL1 was detected in an immunocytochemical section of A549 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.| Product Name | Rabbit polyclonal antibody to Islet 1/ISL1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Islet 1/ISL1 recombinant protein (Position: D118-E161). Human ISL1 shares 100% amino acid (aa) sequence identity with both mouse and rat ISL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-p:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ISL1 |
|---|---|
| Gene Synonyms | Isl-1 ISLET1 |
| Gene Full Name | ISL LIM homeobox 1 |
| Gene Summary | This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008] |
| Alternative Names | Isl 1 ISL LIM homeobox 1 ISL1 Islet 1 ISLET1 |
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Nucleus |
WB
Western blot analysis of anti-Islet 1/ISL1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Islet 1/ISL1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate .
IHC
IHC analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody . Islet 1/ISL1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody. Islet 1/ISL1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody. Islet 1/ISL1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) was used as secondary antibody.
IF-P
IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody. Islet 1/ISL1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Islet 1/ISL1 Antibody at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green)was used as secondary antibody.
ICC/IF
ICC/IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody and anti-Beta Tubulin antibody.Islet 1/ISL1 was detected in an immunocytochemical section of A549 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-p:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
