WB
Western blot analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCDC36/IHO1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. CCDC36/IHO1 was detected in a paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCDC36/IHO1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. CCDC36/IHO1 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCDC36/IHO1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. CCDC36/IHO1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-CCDC36/IHO1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to CCDC36/IHO1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human CCDC36 recombinant protein (Position: E172-N472). Human CCDC36 shares 49% and 51.5% amino acid (aa) sequence identity with mouse and rat CCDC36, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IHO1 |
|---|---|
| Gene Synonyms | CT74 LELA1 CCDC36 |
| Gene Full Name | interactor of HORMAD1 1 |
| Gene Summary | Predicted to be involved in several processes, including meiosis I cell cycle process; regulation of homologous chromosome segregation; and spermatogenesis. Predicted to be located in chromosome. Predicted to be active in condensed nuclear chromosome. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Interactor of HORMAD1 protein 1 CT74 CCDC36 IHO1 |
| Molecular Weight(MW) | 66 kDa |
| Cellular Localization | Chromosome |
WB
Western blot analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCDC36/IHO1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. CCDC36/IHO1 was detected in a paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCDC36/IHO1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. CCDC36/IHO1 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCDC36/IHO1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of CCDC36/IHO1 using anti-CCDC36/IHO1 antibody. CCDC36/IHO1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-CCDC36/IHO1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
