WB
Western Blot: Positive WB detected in Recombinant protein. All lanes: Capsid protein antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.| Product Name | Capsid protein Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Escherichia phage Qbeta (Bacteriophage Q-beta) CAPSD protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:200-1:2000 |
|
| Species Reactivity | Escherichia Coli |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | Capsid protein (CP) (Coat protein) |
|---|---|
| Molecular Weight(MW) | 18.3 kDa(Observed band size: 20 kDa) |
| Function | Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 26 nm in diameter, and consisting of 89 capsid proteins dimers (178 capsid proteins). Involved in viral genome encapsidation through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome. Binding of the capsid proteins to the viral RNA induces a conformational change required for efficient T=3 shell formation. The capsid contains also 1 copy of the A2 maturation protein.; Acts as a translational repressor of viral replicase synthesis late in infection. This latter function is the result of capsid protein interaction with an RNA hairpin which contains the replicase ribosome-binding site. |
| Cellular Localization | Virion. |
WB
Western Blot: Positive WB detected in Recombinant protein. All lanes: Capsid protein antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.| Application Notes | WB:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
